Advanced search Help

Object

Nanoparticle-mediated enhancement of plant cryopreservation: Cultivar-specific insights into morphogenesis and biochemical responses in Lamprocapnos spectabilis (L.) Fukuhara ’Gold Heart’ and ’Valentine’
Download

Title: Nanoparticle-mediated enhancement of plant cryopreservation: Cultivar-specific insights into morphogenesis and biochemical responses in Lamprocapnos spectabilis (L.) Fukuhara ’Gold Heart’ and ’Valentine’

Creator:

Kulus, Dariusz ; Tymoszuk, Alicja ; Kulpińska, Alicja ; Wojnarowicz, Jacek ; Szałaj, Urszula

Date:

2024.05.31

Resource Type:

artykuł

Description:

The integration of nanoparticles (NPs) holds promising potential to bring substantialadvancements to plant cryopreservation, a crucial technique in biodiversity conservation.To date, little attention has been focused on using nanoparticles in cryobiology research.This study aimed to assess the effectiveness of NPs in enhancing the efficiency of plantcryopreservation. In-vitro-derived shoot tips of bleeding heart (Lamprocapnos spectabilis (L.) Fukuhara) ‘Gold Heart’ and ‘Valentine’ were used as the plant material. The encapsulation-vitrification cryopreservation protocol included preculture, encapsulation, dehydration,storage in liquid nitrogen, rewarming, and recovery steps. Gold (AuNPs), silver (AgNPs), orzinc oxide (ZnONPs) nanoparticles were added at various concentrations either into the preculturemedium or the protective bead matrix during encapsulation. The explant survival andfurther morphogenic and biochemical events were studied. Results showed that the impactof NPs on cryopreservation outcomes was cultivar-specific. In the ’Valentine’ cultivar, incorporating5 ppm AgNPs within the alginate bead matrix significantly improved cryopreservationefficiency by up to 12%. On the other hand, the ’Gold Heart’ cultivar benefited fromalginate supplementation with 5 ppm AgNPs and 5-15 ppm ZnONPs, leading to an over28% increase in the survival rate of shoot tips. Interestingly, adding NPs to the preculturemedium was less effective and sometimes counterproductive, despite promoting greatershoot proliferation and elongation in ‘Valentine’ explants compared to the control. Moreover,nanoparticles often induced oxidative stress (and enhanced the activity of APX, GPOX, andSOD enzymes), which in turn affected the biosynthesis of plant primary and secondarymetabolites. It was found that supplementation of preculture medium with higher concentration(15 ppm) of gold, silver and zinc oxide nanoparticles stimulated the production of plantpigments, but in a cultivar-dependent matter. Our study confirmed the beneficial action ofnanoparticles during cryopreservation of plant tissues

Publisher:

PLOS

Resource Identifier:

doi:10.1371/journal.pone.0304586 ; oai:dlibra.pbs.edu.pl:5116

Source:

PLoS ONE 19(5): e0304586

Language:

eng

Relation:

Wydział Rolnictwa i Biotechnologii

License:

Creative Commons by 4.0

Access Rights:

Dla wszystkich zgodnie z powyższą licencją ; click here to follow the link

Subject and keywords:

nanoparticles cryopreservation bleeding heart

Object collections:

Last modified:

Dec 2, 2024

In our library since:

Dec 2, 2024

Number of object content hits:

8

All available object's versions:

https://dlibra.pbs.edu.pl/publication/5182

Show description in RDF format:

RDF

Show description in OAI-PMH format:

OAI-PMH

Edition name Date
Nanoparticle-mediated enhancement of plant cryopreservation: Cultivar-specific insights into morphogenesis and biochemical responses in Lamprocapnos spectabilis (L.) Fukuhara ’Gold Heart’ and ’Valentine’ Dec 2, 2024

Objects Similar
Effect of nanoparticles on the ex-vitro performance of cryopreservation-derived plant material

Kulus, Dariusz Tymoszuk, Alicja Kulpińska, Alicja Viehmannova, Iva Wojnarowicz, Jacek Szałaj, Urszula

2024.09.12
artykuł
Nanoparticles in plant cryopreservation : effects on genetic stability, metabolic profiles, and structural integrity in Bleeding Heart (Papaveraceae) cultivars

Kulus, Dariusz Tymoszuk, Alicja Kulpińska, Alicja Dębska, Bożena (agrotechnika) Michalska, Agata Nowakowska, Julita Wichrowska, Dorota (agronomia) Wojnarowicz, Jacek Szałaj, Urszula

2025.02.17
artykuł
In vitro morphogenesis, cryopreservation and induction of variability in bleeding heart (Lamprocapnos spectabilis (L.) Fukuhara): A review

Kulus, Dariusz

2024.09.12
artykuł
In vitro morphogenesis, cryopreservation and induction of variability in bleeding heart (Lamprocapnos spectabilis (L.) Fukuhara): A review

Kulus, Dariusz

[2024]
preprint
Effect of silver nanoparticles on the in vitro regeneration, biochemical, genetic, and phenotype variation in adventitious shoots produced from leaf explants in chrysanthemum

Tymoszuk, Alicja Kulus, Dariusz

2022.07.03
artykuł
Preparation and properties of silver nanoparticles stabilized by mercaptosuccinic acid with a controllable size

Ścigalski, Franciszek Kaczmarek, Halina Wolnicka, Magdalena

2013
artykuł
Silver-nanoparticles immobilized initiators and co-initiators for free radical polymerization

Ścigalski, Franciszek Jędrzejewska, Beata Pietrzak, Marek Wiśniewski, Waldemar Pączkowski, Jerzy

2011
artykuł
More
×

Citation

Citation style:

Contact details

Address

Biblioteka Główna Politechniki Bydgoskiej
Repozytorium Cyfrowe
Al. prof. S. Kaliskiego 7
85-796 Bydgoszcz, Poland

Phone

(+48) 52 340-8096

E-Mail

repozytorium@pbs.edu.pl

Visit us!

This page uses 'cookies'. More information