Title:

Nanoparticle-mediated enhancement of plant cryopreservation: Cultivar-specific insights into morphogenesis and biochemical responses in Lamprocapnos spectabilis (L.) Fukuhara ’Gold Heart’ and ’Valentine’

Creator:

Kulus, Dariusz ; Tymoszuk, Alicja ; Kulpińska, Alicja ; Wojnarowicz, Jacek ; Szałaj, Urszula

Subject and Keywords:

nanoparticles ; cryopreservation ; bleeding heart

Description:

The integration of nanoparticles (NPs) holds promising potential to bring substantialadvancements to plant cryopreservation, a crucial technique in biodiversity conservation.To date, little attention has been focused on using nanoparticles in cryobiology research.This study aimed to assess the effectiveness of NPs in enhancing the efficiency of plantcryopreservation. In-vitro-derived shoot tips of bleeding heart (Lamprocapnos spectabilis (L.) Fukuhara) ‘Gold Heart’ and ‘Valentine’ were used as the plant material. The encapsulation-vitrification cryopreservation protocol included preculture, encapsulation, dehydration,storage in liquid nitrogen, rewarming, and recovery steps. Gold (AuNPs), silver (AgNPs), orzinc oxide (ZnONPs) nanoparticles were added at various concentrations either into the preculturemedium or the protective bead matrix during encapsulation. The explant survival andfurther morphogenic and biochemical events were studied. Results showed that the impactof NPs on cryopreservation outcomes was cultivar-specific. In the ’Valentine’ cultivar, incorporating5 ppm AgNPs within the alginate bead matrix significantly improved cryopreservationefficiency by up to 12%. On the other hand, the ’Gold Heart’ cultivar benefited fromalginate supplementation with 5 ppm AgNPs and 5-15 ppm ZnONPs, leading to an over28% increase in the survival rate of shoot tips. Interestingly, adding NPs to the preculturemedium was less effective and sometimes counterproductive, despite promoting greatershoot proliferation and elongation in ‘Valentine’ explants compared to the control. Moreover,nanoparticles often induced oxidative stress (and enhanced the activity of APX, GPOX, andSOD enzymes), which in turn affected the biosynthesis of plant primary and secondarymetabolites. It was found that supplementation of preculture medium with higher concentration(15 ppm) of gold, silver and zinc oxide nanoparticles stimulated the production of plantpigments, but in a cultivar-dependent matter. Our study confirmed the beneficial action ofnanoparticles during cryopreservation of plant tissues

Publisher:

PLOS

Date:

2024.05.31

Resource Type:

artykuł

Resource Identifier:

doi:10.1371/journal.pone.0304586

Source:

PLoS ONE 19(5): e0304586

Language:

eng

Relation:

Wydział Rolnictwa i Biotechnologii

License:

Creative Commons by 4.0

Access Rights:

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