Title:

Nanoparticles in plant cryopreservation : effects on genetic stability, metabolic profiles, and structural integrity in Bleeding Heart (Papaveraceae) cultivars

Creator:

Kulus, Dariusz ; Tymoszuk, Alicja ; Kulpińska, Alicja ; Dębska, Bożena (agrotechnika) ; Michalska, Agata ; Nowakowska, Julita ; Wichrowska, Dorota (agronomia) ; Wojnarowicz, Jacek ; Szałaj, Urszula

Subject and Keywords:

nanocząstki ; krioprezerwacja ; serduszka okazała ; nanoparticles ; cryopreservation ; bleedng heart

Description:

Purpose: Studying the role of nanoparticles in plant cryopreservation is essential for developing innovative methods to conserve plantgenetic resources amid environmental challenges. This research investigated the effects of gold (AuNPs), silver (AgNPs), and zincoxide (ZnONPs) nanoparticles on the structural integrity, genetic stability, and metabolic activity of cryopreserved plant materials withmedicinal properties.Methods: Shoot tips from two bleeding heart (Lamprocapnos spectabilis (L). Fukuhara) cultivars, ‘Gold Heart’ and ‘Valentine’, werecryopreserved using the encapsulation-vitrification technique, with nanoparticles added at concentrations of 5 or 15 ppm during eitherthe preculture phase or the alginate bead matrix formation. Post-recovery, the plants underwent histological, molecular, andbiochemical analyses.Results: Electron microscopy observations of LN-derived plant material confirmed the production of micro-morpho-structurallystable cells. It was found that nanoparticles could penetrate the cell and accumulate in its various compartments, including the nucleus.As for the genetic analysis, SCoT markers identified polymorphisms in 11.5% of ‘Gold Heart’ plants, while RAPDs detected mutationsin 1.9% of ‘Valentine’ specimens. Analysis of Molecular Variance (AMOVA) indicated that in the ‘Valentine’ cultivar, all geneticvariation detected was within populations and not significantly affected by nanoparticle treatments. In ‘Gold Heart’, the majority(94%) of genetic variation detected was within populations, while 6% was attributed to nanoparticle treatments (mostly the applicationof 15 ppm ZnONPs). The application of nanoparticles significantly influenced the metabolic profile of bleeding heart plants,particularly affecting the synthesis of phenolic acids and aldehydes, as well as the antioxidant mechanisms in both ‘Gold Heart’and ‘Valentine’ cultivars. The content of proteins was altered in ‘Gold Heart’ plants but not in ‘Valentine’.Conclusion: The results suggest that different types and concentrations of NPs have varying effects on the production of specificmetabolites, which could be harnessed to modulate plant secondary metabolism for desired pharmacological outcomes

Publisher:

Dovepress Taylor and Francis Group

Date:

2025.02.17

Resource Type:

artykuł

Resource Identifier:

doi:10.2147/NSA.S485428

Source:

Nanotechnology, Science and Applications 2025:18 35–56

Language:

eng

Relation:

Wydział Rolnictwa i Biotechnologii

License:

Creative Commons by 4.0

Access Rights:

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